»Year 2021, Volume 14, Pages 1–12
Animal Species Identification of Meat using MALDI-TOF Mass Spectrometry
Jörg Rau1*, Ekkehard Hiller1, Annegret Männig1, Martin Dyk1, Olivera Wenninger1, Philipp Stoll1, Gudrun Wibbelt2 & Pat Schreiter1
1 Chemisches und Veterinäruntersuchungsamt Stuttgart, Fellbach, Germany
2 Leibniz Institute for Zoo and Wildlife Research, 10315 Berlin, Germany
* Corresponding author: Dr. Jörg Rau, email: joerg.rau@cvuas.bwl.de, tel.: +49 711 3426 1264
Available online: 21 December 2021
DOI: 10.48414/aspects2021/14
Keywords: Meat authentication, MALDI-TOF MS, Food fraud, Consumer protection, Animal species, Database, Validation, MALDI-UP (MALDI-User Platform)
Abstract
One of the main aspects of food control regarding meat, seafood and milk products is the inspection and verification of the declared animal species. The potential of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for this purpose has already been demonstrated in principle. In fact, this tool has become an integral part of our official food analysis. In our study we confirm that MALDI-TOF MS is an easy, fast and reliable tool for the identification of animal species when analyzing the meat from pigs, cattle, goats, sheep, horses, turkeys, and chickens. Using a simplified extraction procedure and the Bruker MALDI-Biotyper system, we generated a MALDI-TOF MS database containing more than 550 reference spectra of muscle meat from over 260 confirmed different animal species. In order to accelerate the expansion of this database, we listed the spectra generated in this study on the MALDI-User Platform “MALDI-UP” for exchange with other laboratories.
Supplementary material
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