»Year 2020, Volume 13, Pages 1–13
Collection of Sample Preparation Protocols for MALDI-TOF MS-Based Identification of Meat, Dairy Products, Fish and Insects
Martin Dyk1, Olivera Wenninger1, Christian Guckert2, Jannika Fuchs2, Christine Wind3, Ekkehard Hiller1, Pat Schreiter1 & Jörg Rau1*
1 Chemisches und Veterinäruntersuchungsamt Stuttgart, Fellbach, Germany
2 Chemisches und Veterinäruntersuchungsamt Karlsruhe, Karlsruhe, Germany
3 Chemisches und Veterinäruntersuchungsamt Freiburg, Freiburg, Germany
* Corresponding author, email: joerg.rau@cvuas.bwl.de
Available online: 30 November 2020
DOI: 10.48414/aspects2020/13
Keywords: MALDI-TOF MS, Sample preparation, Species identification, Meat, Dairy products, Fish, Insencts
Abstract
The use of matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF MS) is spreading rapidly in various application areas within food control, veterinary medical diagnostics, clinical microbiology and other fields. One of the most challenging tasks when developing an identification method using MALDI-TOF MS is the extraction of proteins and peptides form the target material. While there are several well-established sample preparation procedures for microorganisms, such as bacteria and filamentous fungi, the users have to develop an suitable sample preparation procedure when applying in areas other than microorganisms. In order to promote the dissemination of the species identification of meat, dairy products, sea foods or insects using MALDI-TOF MS, we provide in this paper a collection of practical protocols for sample preparation, partly based on existing procedures described in literature, with modifications or improvements that have proven successful in our routine food control. These protocols are also uploaded on MALDI-UP that offers the possibility to every MALDI-TOF MS user of sharing his protocols, experience and spectra with other users.
